running gel meaning in English
电泳胶
分离胶
Examples
- The nucleic acids of the all influenza viruses conducted in the research were extracted from the viruses propagated in specific - pathogen - free chicken embryos . all of the eight segments were amplified by rt - pcr , and the purified pcr products were done cycle sequencing with specific primers , furthermore , the sequencing products were purified and run gel on abi prism 377 dna sequencing machine . the specific primers of the eight genes for pcr and cycle sequencing were designed using the ohgo ( 4 . 0 version ) and genedoc ( 2 . 3 version ) software
首先将实验用毒株在spf鸡胚中增殖，提取核酸，然后应用oligo ( 4 . 0版本)和gendoc ( 2 . 3版本)软件设计h9n2aiv所有8个基因片段特异的pcr引物及序列测定引物，通过rt - pcr方法扩增所有毒株的各个基因片段，纯化后用特异引物进行测序反应，反应产物纯化后在abiprism377dna序列分析仪上进行序列测定，然后应用wisconsinsequenceanalysispackage ( gcg ， 10 . 2版本) 、 phylogeneticanalysisusingparsimony ( paup ， 4 . 0版本)和treeview ( 1 . 5版本)软件进行序列的数据编辑、序列翻译、进化树绘制和遗传演化分析。